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| 产品信息 | |
|---|---|
| 描述 | Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed. |
| 别名 | Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor |
| 来源 | Escherichia coli. |
| 分子量 | 6 kd |
| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0. |
| 序列 | NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素水平 | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| 活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存/保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 背景说明 | Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF is a small 53 amino acid residue long protein that contains three disulfide bridges. |
| Accession | P01133 |
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