183***1911
| 产品信息 | |
|---|---|
| 描述 | Recombinant Human Interleukin-6 is produced by our E.coli expression system and the target gene encoding Val30-Met212 is expressed. |
| 别名 | Interleukin-6; IL-6; B-Cell Stimulatory Factor 2; BSF-2; CTL Differentiation Factor; CDF; Hybridoma Growth Factor; Interferon Beta-2; IFN-Beta-2; IL6; IFNB2 |
| 来源 | Escherichia coli. |
| 分子量 | 20.8 kd |
| 形态 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 序列 | PVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLP KMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKA KNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
| 内毒素水平 | Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test. |
| 活性 | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 20-100pg/ml. The ED50 for this effect is typically 79.44 pg/mL. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 储存/保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 背景说明 | Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. |
| Accession | P05231 |
您的位置：
技术支持
datasheet
购物车